YEplac181-TYS1
(Plasmid #8629)

• Depositing Lab: Jeff Strathern
• Publication: Malagon et al Genetics 2004 Mar;166(3):1215-27.

Backbone

• Vector backbone: YEplac181
• Backbone size w/o insert (bp): 5741
• Vector type: Bacterial Expression, Yeast Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TYS1
• Alt name: TTS1
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 2138
• Mutation: Wild type
• Entrez Gene: TYS1 (a.k.a. YGR185C, TTS1)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: PstI (not destroyed)
• 3′ cloning site: SacI (not destroyed)
• 5′ sequencing primer: M13 reverse

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry