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Depositing Lab
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Depositing OrganizationBoston Children's Hospital
Located in the United States of America -
Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 8637 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 8637-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 8637-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepECFP N1
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 4700
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameIntegrin, alpha L
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Alt nameCD11A
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SpeciesH. sapiens (human)
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Insert Size (bp)3500
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GenBank IDNM_002209
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Entrez GeneITGAL (a.k.a. CD11A, LFA-1, LFA1A)
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Tag
/ Fusion Protein
- CFP (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site AgeI (not destroyed)
- 5′ sequencing primer EGFP-N
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note Addgene NGS result identified 3 mutations compared to integrin alpha-L isoform a precursor [Homo sapiens] NP_002200.2: R214W, N770S, P1136S.
DNA (Catalog # 8637-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 8637-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Integrin alpha L - mCFP was a gift from Timothy Springer (Addgene plasmid # 8637 ; http://n2t.net/addgene:8637 ; RRID:Addgene_8637) -
For your References section:
Bidirectional transmembrane signaling by cytoplasmic domain separation in integrins. Kim M, Carman CV, Springer TA. Science 2003 Sep 19;301(5640):1720-5. 10.1126/science.1084174 PubMed 14500982
Ready-to-use antibodies
Looking for antibodies related to this plasmid? Check out this option:
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Map uploaded by the depositor.
