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pSTAP-seq_fly-zfh1_spikeIn-2
(Plasmid #86388)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 86388 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGL3
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 3426
  • Vector type
    STAP-seq spike in vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    D. melanogaster zfh1 enhancer & D. pseudoobscura CG32369 core promoter
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    237

Cloning Information

Resource Information

Depositor Comments

here we inserted a single sequence (rather then a library) into pSTAP-seq_fly-zfh1 between AgeI and SalI using In-Fusion (Clonetech)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSTAP-seq_fly-zfh1_spikeIn-2 was a gift from Alexander Stark (Addgene plasmid # 86388 ; http://n2t.net/addgene:86388 ; RRID:Addgene_86388)
  • For your References section:

    Genome-wide assessment of sequence-intrinsic enhancer responsiveness at single-base-pair resolution. Arnold CD, Zabidi MA, Pagani M, Rath M, Schernhuber K, Kazmar T, Stark A. Nat Biotechnol. 2016 Dec 26. doi: 10.1038/nbt.3739. 10.1038/nbt.3739 PubMed 28024147