PurposeExpression vector for a transcript origination from a D. pse core promoter, which is used as trasfection control (normalization), i.e. STAP-seq screening vector containing one specific core promoter
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||86389||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3426
Vector typeSTAP-seq spike in vector
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameD. melanogaster zfh1 enhancer & D. pseudoobscura ush core promoter -TSS#1
SpeciesD. melanogaster (fly)
Insert Size (bp)237
- Cloning method Gibson Cloning
- 5′ sequencing primer RVprimer3 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
here we inserted a single sequence (rather then a library) into pSTAP-seq_fly-zfh1 between AgeI and SalI using In-Fusion (Clonetech)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pSTAP-seq_fly-zfh1_spikeIn-3 was a gift from Alexander Stark (Addgene plasmid # 86389 ; http://n2t.net/addgene:86389 ; RRID:Addgene_86389)
For your References section:Genome-wide assessment of sequence-intrinsic enhancer responsiveness at single-base-pair resolution. Arnold CD, Zabidi MA, Pagani M, Rath M, Schernhuber K, Kazmar T, Stark A. Nat Biotechnol. 2016 Dec 26. doi: 10.1038/nbt.3739. 10.1038/nbt.3739 PubMed 28024147