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pSJ1321 (pRS315-NOP1pr-GFP11-mCherry-PUS1)
(Plasmid #86413)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 86413 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pRS315
  • Backbone size w/o insert (bp) 5000
  • Total vector size (bp) 10535
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Nop1pr-GFP11-mCherry-PUS1
  • Species
    S. cerevisiae (budding yeast), Synthetic
  • Promoter NOP1
  • Tags / Fusion Proteins
    • mCherry (C terminal on insert)
    • GFP11 (C terminal on insert)

Cloning Information

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSJ1321 (pRS315-NOP1pr-GFP11-mCherry-PUS1) was a gift from Sue Jaspersen (Addgene plasmid # 86413 ; http://n2t.net/addgene:86413 ; RRID:Addgene_86413)
  • For your References section:

    Analysis of membrane proteins localizing to the inner nuclear envelope in living cells. Smoyer CJ, Katta SS, Gardner JM, Stoltz L, McCroskey S, Bradford WD, McClain M, Smith SE, Slaughter BD, Unruh JR, Jaspersen SL. J Cell Biol. 2016 Nov 21;215(4):575-590. Epub 2016 Nov 9. 10.1083/jcb.201607043 PubMed 27831485