pmCherry-GFP
(Plasmid
#86441)
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Purposevisualization and quantifying the out-of-frame mutations from genome editing
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Depositing Lab
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Depositing OrganizationNational Institute of Child Health and Human Development (NICHD)
Located in the United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 86441 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 86441-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 86441-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepUC19
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Backbone manufacturerThermofischer
- Backbone size w/o insert (bp) 2687
- Total vector size (bp) 3963
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Vector typeColor visualization in bacteria
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemCherry-MCS-sfGFP fusion gene
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Insert Size (bp)1530
- Promoter Plac
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Tag
/ Fusion Protein
- mCherry-sfGFP
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site NdeI (destroyed during cloning)
- 5′ sequencing primer M13R
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made bymCherry was PCR-amplified from Addgene clone#59995; sfGFP gene was synthesized based on sequences of GenBank # HQ873313. sfGFP gene was synthesized based on sequences of GenBank # HQ873313.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The whole plasmid sequences were compiled based on vector sequences information. It was not confirmed by DNA sequencing.
DNA (Catalog # 86441-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 86441-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pmCherry-GFP was a gift from Yun-Bo Shi (Addgene plasmid # 86441 ; http://n2t.net/addgene:86441 ; RRID:Addgene_86441) -
For your References section:
A simple and efficient method to visualize and quantify the efficiency of chromosomal mutations from genome editing. Fu L, Wen L, Luu N, Shi YB. Sci Rep. 2016 Oct 17;6:35488. doi: 10.1038/srep35488. 10.1038/srep35488 PubMed 27748423
Map uploaded by the depositor.
