pCAM45
(Plasmid
#86485)
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PurposeExpresses csoS1A-mRFP1
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 86485 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepWW307
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Modifications to backboneconstitutive mRFP1 on the backbone is fused to the C terminus of csoS1A
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namecsoS1A-mRFP1
- Promoter pBAD
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Tag
/ Fusion Protein
- mRFP1 (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer pBAD Forward from Eton Bio
- 3′ sequencing primer pBAD Reverse from Eton Bio (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCAM45 was a gift from Pamela Silver (Addgene plasmid # 86485 ; http://n2t.net/addgene:86485 ; RRID:Addgene_86485) -
For your References section:
A distributed cell division counter reveals growth dynamics in the gut microbiota. Myhrvold C, Kotula JW, Hicks WM, Conway NJ, Silver PA. Nat Commun. 2015 Nov 30;6:10039. doi: 10.1038/ncomms10039. 10.1038/ncomms10039 PubMed 26615910
