Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pCAM72
(Plasmid #86488)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 86488 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pWW307
  • Modifications to backbone
    constitutive mRFP1 on the backbone is fused to the C terminus of T4_gp23
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DP10
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    T4_gp23-mRFP1
  • Promoter pBAD
  • Tag / Fusion Protein
    • mRFP1 (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer pBAD Forward from Eton Bio
  • 3′ sequencing primer pBAD Reverse from Eton Bio
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAM72 was a gift from Pamela Silver (Addgene plasmid # 86488 ; http://n2t.net/addgene:86488 ; RRID:Addgene_86488)
  • For your References section:

    A distributed cell division counter reveals growth dynamics in the gut microbiota. Myhrvold C, Kotula JW, Hicks WM, Conway NJ, Silver PA. Nat Commun. 2015 Nov 30;6:10039. doi: 10.1038/ncomms10039. 10.1038/ncomms10039 PubMed 26615910