CIAR_pcDNA5/FRT/TO
(Plasmid
#86502)
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PurposeExpresses CIAR in mammalian cells. Used to generate Flp-In stables.
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Depositing Lab
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Depositing OrganizationUniversity of Washington
Located in the United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 86502 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 86502-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 86502-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepcDNA5/FRT/TO
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5137
- Total vector size (bp) 7339
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Vector typeMammalian Expression ; FRT
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCIAR
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Alt nameS.17.3
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Alt nameChemically Inducible Activator of RAS
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SpeciesH. sapiens (human)
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Insert Size (bp)2202
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MutationT968L in SOScat
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Entrez GeneSOS1 (a.k.a. GF1, GGF1, GINGF, HGF, NS4, SOS-1)
- Promoter CMV (tet operator)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV_F
- 3′ sequencing primer BGHR
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 86502-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 86502-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CIAR_pcDNA5/FRT/TO was a gift from Dustin Maly (Addgene plasmid # 86502 ; http://n2t.net/addgene:86502 ; RRID:Addgene_86502) -
For your References section:
A computationally engineered RAS rheostat reveals RAS-ERK signaling dynamics. Rose JC, Huang PS, Camp ND, Ye J, Leidal AM, Goreshnik I, Trevillian BM, Dickinson MS, Cunningham-Bryant D, Debnath J, Baker D, Wolf-Yadlin A, Maly DJ. Nat Chem Biol. 2016 Nov 21. doi: 10.1038/nchembio.2244. 10.1038/nchembio.2244 PubMed 27870838
