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Ubiquitin K63C/G76C
(Plasmid #86586)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 86586 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Ubiquitin
  • Species
    H. sapiens (human)
  • Mutation
    TEV site; K63C/G76C
  • Entrez Gene
    UBC (a.k.a. HMG20)
  • Promoter Tac
  • Tag / Fusion Protein
    • His6-MBP (N terminal on backbone)

Cloning Information

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Ubiquitin K63C/G76C was a gift from Sharon Rozovsky (Addgene plasmid # 86586 ; http://n2t.net/addgene:86586 ; RRID:Addgene_86586)
  • For your References section:

    Utilizing Selenocysteine for Expressed Protein Ligation and Bioconjugations. Liu J, Chen Q, Rozovsky S. J Am Chem Soc. 2017 Feb 10. doi: 10.1021/jacs.6b10991. 10.1021/jacs.6b10991 PubMed 28186733