MTST6
(Plasmid
#86603)
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PurposeCustom designed synthetic DNA fragment lacking homology to natural sequences for preparation of internal standards for RNA-seq in metatranscriptomics.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 86603 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
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Backbone
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Vector backbonepUC57
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Backbone manufacturerGenewiz
- Backbone size w/o insert (bp) 2700
- Total vector size (bp) 3700
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameMTST6
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Alt nameMTST6
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SpeciesSynthetic
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Insert Size (bp)1006
- Promoter T7
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRV (unknown if destroyed)
- 3′ cloning site PmeI (unknown if destroyed)
- 5′ sequencing primer GTAAAACGACGGCCAGTG
- 3′ sequencing primer GGAAACAGCTATGACCATG (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MTST6 was a gift from Mary Ann Moran (Addgene plasmid # 86603 ; http://n2t.net/addgene:86603 ; RRID:Addgene_86603) -
For your References section:
Use of internal standards for quantitative metatranscriptome and metagenome analysis. Satinsky BM, Gifford SM, Crump BC, Moran MA. Methods Enzymol. 2013;531:237-50. doi: 10.1016/B978-0-12-407863-5.00012-5. 10.1016/B978-0-12-407863-5.00012-5 PubMed 24060124
Map uploaded by the depositor.
