CDk4-luciferase fragment 3
PurposeLuciferase reporter containing CDK4 promoter
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||86662||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Gene/Insert nameCDK4 promoter
SpeciesH. sapiens (human)
Mutationgenerated from 880 -416 to -658
Entrez GeneCDK4 (a.k.a. CMM3, PSK-J3)
- Promoter CDK10
/ Fusion Protein
- luciferase (C terminal on backbone)
- Cloning method Unknown
- 5′ sequencing primer RVprimer3
- 3′ sequencing primer LucNrev (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Addgene NGS found a single nucleotide mismatch compared to reference sequence AY034380; however depositing lab confirms plasmid functionality
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:CDk4-luciferase fragment 3 was a gift from Jaime Modiano (Addgene plasmid # 86662 ; http://n2t.net/addgene:86662 ; RRID:Addgene_86662)