pRB2065
(Plasmid #8669)

• Depositing Lab: David Botstein
• Publication: Richards et al Mol Biol Cell 2000 May;11(5):1887-903.

Backbone

• Vector backbone: pUC119
• Vector type: Yeast Expression
• Selectable markers: LEU2

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Unknown
• Growth instructions: Don't know
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: TUB1 upstream of LEU2
• Alt name: TUB1
• Species: S. cerevisiae (budding yeast)
• Entrez Gene: TUB1 (a.k.a. YML085C)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: See comments (unknown if destroyed)
• 3′ cloning site: See comments (unknown if destroyed)
• 5′ sequencing primer: N/A

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

TUB1 fragment (SphI-SacI) from pRB306 inserted into SphI-SacI-cut polylinker of pUC119.
Then, BamHI-SalI fragment from pJJ282 (LEU2 gene) Klenow filled and inserted into the HpaI site, downstream of TUB1; LEU2 is oriented in the same direction as TUB1. For information on pUC119, search for pUC119 in Addgene's vector db.