Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

mCherry-UVRAG S498A
(Plasmid #86744)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 86744 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pmCherry-1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    human UVRAG
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2100
  • Mutation
    S498A
  • Entrez Gene
    UVRAG (a.k.a. DHTX, VPS38, p63)
  • Promoter CMV
  • Tag / Fusion Protein
    • mCherry (N terminal on backbone)

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

IMAGE: 6186859

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mCherry-UVRAG S498A was a gift from Do-Hyung Kim (Addgene plasmid # 86744 ; http://n2t.net/addgene:86744 ; RRID:Addgene_86744)
  • For your References section:

    mTORC1 phosphorylates UVRAG to negatively regulate autophagosome and endosome maturation. Kim YM, Jung CH, Seo M, Kim EK, Park JM, Bae SS, Kim DH. Mol Cell. 2015 Jan 22;57(2):207-18. doi: 10.1016/j.molcel.2014.11.013. Epub 2014 Dec 18. 10.1016/j.molcel.2014.11.013 PubMed 25533187