HOXC13(human)-pET
(Plasmid #8679)

• Depositing Lab: Corey Largman
• Publication: Komuves et al Dev Dyn 2003 Jun;227(2):192-202.

Backbone

• Vector backbone: pET28a
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5400
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: HOXC13
• Alt name: HOX3G
• Species: H. sapiens (human)
• Insert Size (bp): 995
• Mutation: makes protein by Coomassie blue staining
• GenBank ID: NM_017410
• Entrez Gene: HOXC13 (a.k.a. ECTD9, HOX3, HOX3G)
• Tags / Fusion Proteins:
  • His (N terminal on backbone)
  • T7 (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XhoI (destroyed during cloning)
• 5′ sequencing primer: T7

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Alexander Awgulewitsch

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Tkatchenko et al Development 128: 1547-1558 (2001)