pD603 H77 E1E2
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||86983||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerDNA 2.0
Vector typeMammalian Expression
Growth in Bacteria
Growth Strain(s)NEB Stable
Copy numberHigh Copy
Gene/Insert nameHCV E1E2
- Promoter CMV
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Terms and Licenses
Note: The HCV E1E2 insert contains an N391S mutation compared to AAB67036.1. The encoded protein is based on the consensus H77 sequence (AF009606), as described in https://www.ncbi.nlm.nih.gov/pubmed/9228008.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pD603 H77 E1E2 was a gift from Joe Grove (Addgene plasmid # 86983)