jk107
(Plasmid
#87007)
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Purposeplasmid for cell culture expression of SYNseq components
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Depositing Lab
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Depositing OrganizationCold Spring Harbor Laboratory
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 87007 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 87007-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 87007-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepSinEGdsp
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Vector typeMammalian Expression, Synthetic Biology ; SIndbis virus genomic plasmid
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert 1
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Gene/Insert nameSNAP-Nlg1AB-1xλN(i)
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SpeciesM. musculus (mouse)
Cloning Information for Gene/Insert 1
- Cloning method Gibson Cloning
- 5′ sequencing primer unknown
- (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert namemCherry
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 87007-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 87007-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
jk107 was a gift from Anthony Zador (Addgene plasmid # 87007 ; http://n2t.net/addgene:87007 ; RRID:Addgene_87007) -
For your References section:
Using high-throughput barcode sequencing to efficiently map connectomes. Peikon ID, Kebschull JM, Vagin VV, Ravens DI, Sun YC, Brouzes E, Correa IR Jr, Bressan D, Zador AM. Nucleic Acids Res. 2017 Jul 7;45(12):e115. doi: 10.1093/nar/gkx292. 10.1093/nar/gkx292 PubMed 28449067