pET28b-pchA WT
(Plasmid #87102)

• Purpose: expresses PchA WT, the isochorismate synthase from Pseudomonas aeruginosa
• Depositing Lab: Audrey Lamb
• Publication: Meneely et al Arch Biochem Biophys. 2013 Oct 1;538(1):49-56. doi: 10.1016/j.abb.2013.07.026. Epub 2013 Aug 11.

Backbone

• Vector backbone: pET28b
• Backbone manufacturer: novagen
• Backbone size w/o insert (bp): 5368
• Total vector size (bp): 6799
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: Room Temperature
• Growth Strain(s): DH5alpha
• Growth instructions: Grown in terrific broth with 0.4% glycerol for 22 hours after induction.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: pchA WT
• Species: Pseudomonas aeruginosa PAO1
• Insert Size (bp): 1431
• Promoter: T7
• Tag / Fusion Protein:
  • his-tag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: pET upstream
• 3′ sequencing primer: T7 reverse

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Archives of Biochemistry and Biophysics, 539 (2013) 70-80, PMC3836504
JACS 138 (2016) 9277-9293, PMC 5029964