pCDH1-hPRMT7-GFP
(Plasmid
#87103)
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Purposeexpress hPRMT7 in mammalian cells
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Depositing Lab
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Depositing OrganizationUniversity of Texas MD Anderson Cancer Center
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 87103 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 87103-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 87103-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepCDH-EF1-IRES-GFP
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Backbone manufacturerSystem Biosciences
- Backbone size w/o insert (bp) 7200
- Total vector size (bp) 9300
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameProtein arginine methyltransferase 7
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SpeciesH. sapiens (human)
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Insert Size (bp)2079
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Entrez GenePRMT7 (a.k.a. SBIDDS)
- Promoter EF1
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer EF1 sequencing primer
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 87103-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 87103-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCDH1-hPRMT7-GFP was a gift from Min Gyu Lee (Addgene plasmid # 87103 ; http://n2t.net/addgene:87103 ; RRID:Addgene_87103) -
For your References section:
A feedback loop comprising PRMT7 and miR-24-2 interplays with Oct4, Nanog, Klf4 and c-Myc to regulate stemness. Lee SH, Chen TY, Dhar SS, Gu B, Chen K, Kim YZ, Li W, Lee MG. Nucleic Acids Res. 2016 Dec 15;44(22):10603-10618. Epub 2016 Sep 12. 10.1093/nar/gkw788 PubMed 27625395
