pET28b-irp3 WT
(Plasmid
#87106)
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Purposeexpresses Irp3 WT, the thiazolinyl imine reductase from Yersinia enterocolitica
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 87106 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepET28b
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Backbone manufacturernovagen
- Backbone size w/o insert (bp) 5368
- Total vector size (bp) 6466
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth TemperatureRoom Temperature
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Growth Strain(s)DH5alpha
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Growth instructions18 hours growth after induction
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameirp3 WT
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SpeciesYersinia enterocolitica
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Insert Size (bp)1098
- Promoter T7
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Tag
/ Fusion Protein
- his-tag (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer pET upstream
- 3′ sequencing primer T7 reverse (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Biochemistry 55 (2016) 5423-5433
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET28b-irp3 WT was a gift from Audrey Lamb (Addgene plasmid # 87106 ; http://n2t.net/addgene:87106 ; RRID:Addgene_87106) -
For your References section:
Two structures of a thiazolinyl imine reductase from Yersinia enterocolitica provide insight into catalysis and binding to the nonribosomal peptide synthetase module of HMWP1. Meneely KM, Lamb AL. Biochemistry. 2012 Nov 6;51(44):9002-13. doi: 10.1021/bi3011016. Epub 2012 Oct 23. 10.1021/bi3011016 PubMed 23066849
