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pGL2-MCT2Prom0.6
(Plasmid #87353)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 87353 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 87353-D50 50 μg of DNA in Tris buffer $495
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DNA 87353-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pGL2
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 5598
  • Total vector size (bp) 6137
  • Vector type
    Mammalian Expression, Bacterial Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable ; E. coli SURE for propagation, E. coli SCS110 for reporter studies
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MCT2
  • Alt name
    SLC16A7
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    539
  • Entrez Gene
    SLC16A7 (a.k.a. MCT2)
  • Promoter 0.6 kbp proximal promoter of human MCT2 (nested deletion construct)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (destroyed during cloning)
  • 3′ cloning site HinDIII (not destroyed)
  • 5′ sequencing primer GL primer 1
  • 3′ sequencing primer GL primer 2
  • (Common Sequencing Primers)

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

A methylation deficient E. coli strain such as SCS110 is preferred to prepare plasmid DNA for mammalian cell transfection to reduce affects of prokaryotic methylation patterns on promoter activity.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL2-MCT2Prom0.6 was a gift from Saroj Mathupala (Addgene plasmid # 87353 ; http://n2t.net/addgene:87353 ; RRID:Addgene_87353)

  • For your References section:

    pH, Lactate, and Hypoxia: Reciprocity in Regulating High-Affinity Monocarboxylate Transporter Expression in Glioblastoma. Caruso JP, Koch BJ, Benson PD, Varughese E, Monterey MD, Lee AE, Dave AM, Kiousis S, Sloan AE, Mathupala SP. Neoplasia. 2017 Jan 13;19(2):121-134. doi: 10.1016/j.neo.2016.12.011. 10.1016/j.neo.2016.12.011 PubMed 28092823
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