NCMV MmuPV1 E6 R52A
(Plasmid
#87678)
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PurposeFlag/HA tagged MmuPV1 E6 R52A
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 87678 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backboneCMV Bam neo
- Backbone size w/o insert (bp) 9387
- Total vector size (bp) 8600
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Vector typeLentiviral
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMmuPV1 E6
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SpeciesMmuPV1
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Insert Size (bp)422
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MutationR52A
- Promoter CMV
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Tags
/ Fusion Proteins
- Flag (N terminal on insert)
- HA (N terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer CMV forward
- 3′ sequencing primer V5 Reverse (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
NCMV MmuPV1 E6 R52A was a gift from Karl Munger (Addgene plasmid # 87678 ; http://n2t.net/addgene:87678 ; RRID:Addgene_87678) -
For your References section:
Cutaneous HPV8 and MmuPV1 E6 Proteins Target the NOTCH and TGF-beta Tumor Suppressors to Inhibit Differentiation and Sustain Keratinocyte Proliferation. Meyers JM, Uberoi A, Grace M, Lambert PF, Munger K. PLoS Pathog. 2017 Jan 20;13(1):e1006171. doi: 10.1371/journal.ppat.1006171. PPATHOGENS-D-16-02140 [pii] PubMed 28107544
