pFLAG-CMV2 MAML LHHLL
(Plasmid
#87681)
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PurposeMammalian expression vector for Maml1 with LHHLL mutation
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Depositing Lab
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Depositing OrganizationTufts University
Located in the United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 87681 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 87681-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 87681-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepFLAG-CMV2
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Backbone manufacturerSigma
- Backbone size w/o insert (bp) 4679
- Total vector size (bp) 7727
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMAML1
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SpeciesH. sapiens (human)
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Insert Size (bp)3048
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MutationMutate 1010,1011 from DD to HH
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Entrez GeneMAML1 (a.k.a. Mam-1, Mam1)
- Promoter CMV
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Tag
/ Fusion Protein
- Flag (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SalI (unknown if destroyed)
- 3′ cloning site NotI (unknown if destroyed)
- 5′ sequencing primer CMV forward
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byOriginal template DNA was a gift of J. Aster.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Additional mutation G162C within the MAML1 translation was in the original CMV2 Flag MAML plasmid we were given by the J. Aster lab
DNA (Catalog # 87681-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 87681-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFLAG-CMV2 MAML LHHLL was a gift from Karl Munger (Addgene plasmid # 87681 ; http://n2t.net/addgene:87681 ; RRID:Addgene_87681) -
For your References section:
Cutaneous HPV8 and MmuPV1 E6 Proteins Target the NOTCH and TGF-beta Tumor Suppressors to Inhibit Differentiation and Sustain Keratinocyte Proliferation. Meyers JM, Uberoi A, Grace M, Lambert PF, Munger K. PLoS Pathog. 2017 Jan 20;13(1):e1006171. doi: 10.1371/journal.ppat.1006171. PPATHOGENS-D-16-02140 [pii] PubMed 28107544
