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PurposeExpresses IDH2 (R172K mutant) in mammalian cells
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Depositing Lab
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Depositing OrganizationCenter for Open Science
Located in the United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 87927 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 87927-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 87927-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepcDNA3.1
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Backbone manufacturerInvitrogen, cat# V790-20
- Backbone size w/o insert (bp) 5428
- Total vector size (bp) 6800
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameisocitrate dehydrogenase (NADP(+)) 2, mitochondrial
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Alt nameIDH2
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SpeciesH. sapiens (human)
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Insert Size (bp)1359
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MutationR172K
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GenBank IDNM_002168.3
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Entrez GeneIDH2 (a.k.a. D2HGA2, ICD-M, IDH, IDH-2, IDHM, IDP, IDPM, mNADP-IDH)
- Promoter CMV
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Tag
/ Fusion Protein
- none
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer T7 Forward
- 3′ sequencing primer BGH Reverse
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byIDH2 R172K was amplified by PCR from pCMV6-Entry vectors (Origene, cat# RC400103
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 87927-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 87927-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1-IDH2-R172K was a gift from Reproducibility Project Cancer Biology (Addgene plasmid # 87927 ; http://n2t.net/addgene:87927 ; RRID:Addgene_87927) -
For your References section:
Replication Study: The common feature of leukemia-associated IDH1 and IDH2 mutations is a neomorphic enzyme activity converting alpha-ketoglutarate to 2-hydroxyglutarate. Showalter MR, Hatakeyama J, Cajka T, VanderVorst K, Carraway KL, Fiehn O. Elife. 2017 Jun 27;6. pii: e26030. doi: 10.7554/eLife.26030. 10.7554/eLife.26030 PubMed 28653623
