pET21a_Msm_RbpA
(Plasmid #87928)

• Purpose: Mycobacterium smegmatis RpbA in pET21a (ampR) used to co-express with Msm SigA for crystallization purposes
• Depositing Lab: Seth Darst
• Publication: Hubin et al Elife. 2017 Jan 9;6. pii: e22520. doi: 10.7554/eLife.22520.

Backbone

• Vector backbone: pET21a
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5443
• Total vector size (bp): 5788
• Modifications to backbone: none
• Vector type: Bacterial Expression
• Selectable markers: ampicillin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: To express use BL21 (DE3) strains
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: RbpA
• Species: Mycobacterium smegmatis
• Insert Size (bp): 345
• GenBank ID: HQ203032.1
• Promoter: T7P

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (unknown if destroyed)
• 3′ cloning site: BamHI (unknown if destroyed)
• 5′ sequencing primer: t7p
• 3′ sequencing primer: t7t

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry