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pET26b-Granzyme A
(Plasmid #8823)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 8823 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET26b(+)
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5360
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    For protein expression, use only BL21(DE3) competent cell from Novagen
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Human Granzyme A
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    716
  • Mutation
    it mature enzyme.
  • GenBank ID
    NM_006144
  • Entrez Gene
    GZMA (a.k.a. CTLA3, HFSP)
  • Tags / Fusion Proteins
    • (His)6 (C terminal on backbone)
    • Enterokinase site (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site Xho I (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 terminator
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET26b-Granzyme A was a gift from Judy Lieberman (Addgene plasmid # 8823 ; http://n2t.net/addgene:8823 ; RRID:Addgene_8823)
  • For your References section:

    Recombinant human granzyme A binds to two putative HLA-associated proteins and cleaves one of them. Beresford PJ, Kam CM, Powers JC, Lieberman J. Proc Natl Acad Sci U S A 1997 Aug 19;94(17):9285-90. 10.1073/pnas.94.17.9285 PubMed 9256474