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pPS934
(Plasmid #8855)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 8855 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pRS314
  • Backbone size w/o insert (bp) 4800
  • Vector type
    Yeast Expression
  • Selectable markers
    TRP1

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    NUF2-sGFP
  • Alt name
    pJK52
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    2500
  • Entrez Gene
    NUF2
  • Tag / Fusion Protein
    • GFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer M13R
  • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

CEN plasmid. NUF2 promoter, NUF2 ORF, sGFP, NUF2 3' UTR. See author's map.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPS934 was a gift from Pamela Silver (Addgene plasmid # 8855 ; http://n2t.net/addgene:8855 ; RRID:Addgene_8855)
  • For your References section:

    The yeast dynactin complex is involved in partitioning the mitotic spindle between mother and daughter cells during anaphase B. Kahana JA, Schlenstedt G, Evanchuk DM, Geiser JR, Hoyt MA, Silver PA. Mol Biol Cell. 1998 Jul . 9(7):1741-56. 10.1091/mbc.9.7.1741 PubMed 9658168