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3788 pmko-neo A9-3
(Plasmid #8880)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 8880 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pmko-neo
  • Backbone size w/o insert (bp) 7200
  • Vector type
    Mammalian Expression, Retroviral, RNAi
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Hox A9
  • Alt name
    shRNA-HoxA9
  • Species
    H. sapiens (human)
  • Entrez Gene
    HOXA9 (a.k.a. ABD-B, HOX1, HOX1.7, HOX1G)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pLXSN 5'
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

Sequence in hairpin: TGGTT CTCCT CCAGT TGATA.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    3788 pmko-neo A9-3 was a gift from Stanley Korsmeyer (Addgene plasmid # 8880 ; http://n2t.net/addgene:8880 ; RRID:Addgene_8880)
  • For your References section:

    Conditional MLL-CBP targets GMP and models therapy-related myeloproliferative disease. Wang J, Iwasaki H, Krivtsov A, Febbo PG, Thorner AR, Ernst P, Anastasiadou E, Kutok JL, Kogan SC, Zinkel SS, Fisher JK, Hess JL, Golub TR, Armstrong SA, Akashi K, Korsmeyer SJ. EMBO J. 2005 Jan 26. 24(2):368-81. 10.1038/sj.emboj.7600521 PubMed 15635450