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pPS311
(Plasmid #8903)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 8903 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    YCp50 LEU
  • Backbone size w/o insert (bp) 8000
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    GAL1 promoter
  • Alt name
    YCpGAL1 LEU
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    800
  • Entrez Gene
    GAL1 (a.k.a. YBR020W)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (destroyed during cloning)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer na
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

GAL1 promoter from fragment of pPS293, CEN plasmid. See author's map.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPS311 was a gift from Pamela Silver (Addgene plasmid # 8903 ; http://n2t.net/addgene:8903 ; RRID:Addgene_8903)
  • For your References section:

    A yeast RNA-binding protein shuttles between the nucleus and the cytoplasm. Flach J, Bossie M, Vogel J, Corbett A, Jinks T, Willins DA, Silver PA. Mol Cell Biol. 1994 Dec . 14(12):8399-407. PubMed 7969175