pPS311
(Plasmid
#8903)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 8903 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backboneYCp50 LEU
- Backbone size w/o insert (bp) 8000
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Vector typeYeast Expression
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Selectable markersLEU2
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameGAL1 promoter
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Alt nameYCpGAL1 LEU
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)800
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Entrez GeneGAL1 (a.k.a. YBR020W)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (destroyed during cloning)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer na (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
GAL1 promoter from fragment of pPS293, CEN plasmid. See author's map.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPS311 was a gift from Pamela Silver (Addgene plasmid # 8903 ; http://n2t.net/addgene:8903 ; RRID:Addgene_8903) -
For your References section:
A yeast RNA-binding protein shuttles between the nucleus and the cytoplasm. Flach J, Bossie M, Vogel J, Corbett A, Jinks T, Willins DA, Silver PA. Mol Cell Biol. 1994 Dec . 14(12):8399-407. PubMed 7969175
Map uploaded by the depositor.
