Purposemammalian expression of full-length human p300 in pSG5
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||89094||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Entrez GeneEP300 (a.k.a. KAT3B, MKHK2, RSTS2, p300)
- Promoter SV40
/ Fusion Protein
- HA (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (destroyed during cloning)
- 3′ cloning site BamHI (destroyed during cloning)
- 5′ sequencing primer T7
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Addgene's sequencing results found a D1507N mutation in p300 compared to NCBI reference sequence NM_001429.3. This mutation is not known to affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pSG5-HA-p300 was a gift from Elizabeth Wilson (Addgene plasmid # 89094 ; http://n2t.net/addgene:89094 ; RRID:Addgene_89094)
For your References section:Transcriptional synergy between melanoma antigen gene protein-A11 (MAGE-11) and p300 in androgen receptor signaling. Askew EB, Bai S, Blackwelder AJ, Wilson EM. J Biol Chem. 2010 Jul 9;285(28):21824-36. doi: 10.1074/jbc.M110.120600. Epub 2010 May 6. 10.1074/jbc.M110.120600 PubMed 20448036