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pCIneo-hCHD7
(Plasmid #89458)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 89458 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCIneo
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 5470
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CHD7
  • Alt name
    chromodomain helicase DNA binding protein 7
  • Alt name
    NC_00008
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    9230
  • Entrez Gene
    CHD7 (a.k.a. CRG, HH5, IS3, KAL5)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI/XbaI (destroyed during cloning)
  • 3′ cloning site SwaI/SmaI (destroyed during cloning)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T3
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

includes th enative ATG context and native polyA adenylation signal. Note: polymorphic or introduced gga>ggg at Gly360. mutated cct>cca of Pro 481 to create PflMI site. Mutated ttc>ttt of Phe 1035 to create BstBI site

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCIneo-hCHD7 was a gift from Peter Scacheri (Addgene plasmid # 89458 ; http://n2t.net/addgene:89458 ; RRID:Addgene_89458)