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pLenti-PRSX8 eArchT3.0-EYFP
(Plasmid #89538)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 89538 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pLenti
  • Backbone size w/o insert (bp) 9700
  • Modifications to backbone
    insertion of PRSX8 promoter
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    eArchT 3.0
  • Alt name
    Archaerhodopsin
  • Species
    Synthetic
  • Promoter PRSx8
  • Tag / Fusion Protein
    • EYFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PacI (not destroyed)
  • 3′ cloning site BsrGI (not destroyed)
  • 5′ sequencing primer ACG CGT ATA AGC TTC CGC TAG ACA
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Based on pLenti-CaMKIIa-eArchT 3.0-EYFP from Karl Deisseroth

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti-PRSX8 eArchT3.0-EYFP was a gift from Ruth Stornetta (Addgene plasmid # 89538 ; http://n2t.net/addgene:89538 ; RRID:Addgene_89538)
  • For your References section:

    State-dependent control of breathing by the retrotrapezoid nucleus. Burke PG, Kanbar R, Basting TM, Hodges WM, Viar KE, Stornetta RL, Guyenet PG. J Physiol. 2015 Jul 1;593(13):2909-26. doi: 10.1113/JP270053. Epub 2015 May 22. 10.1113/JP270053 PubMed 25820491