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Lenti-dCas9-KRAB-blast Notes

Addgene Diagnostic Digest

This gel depicts the NEB 1kb extended ladder and a digestion of the plasmid Lenti-dCas9-KRAB-blast with the following enzymes: PmeI and SpeI-HF.

Expected Bands:
PmeI 14 kb
SpeI-HF 14 kb
PmeI/ Spel-HF 10 kb & 4.3 kb

These results show the expected pattern.