PurposeAllelic change mutagenesis
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||89632||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerPMID: 9380741
- Backbone size w/o insert (bp) 8550
- Total vector size (bp) 9681
Vector typeAllelic change mutagenesis
Growth in Bacteria
Alt namecatechol 2,3-dioxygenase
- Promoter Pmyco
- Cloning method Restriction Enzyme
- 5′ cloning site PstI (not destroyed)
- 3′ cloning site PstI (not destroyed)
- 5′ sequencing primer unknown (Common Sequencing Primers)
Terms and Licenses
SacB is used for counter-selecton in Mycobacteria to force excision and loss of this plasmid to occur.
This plasmid has been used as an allelic exchange vector. We have used SpeI site for cloning of an insert (flanking regions of a target gene to be deleted), so other groups can also use SpeI (NotI and XbaI can be used as well).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pX33 was a gift from Greg Cook (Addgene plasmid # 89632 ; http://n2t.net/addgene:89632 ; RRID:Addgene_89632)
For your References section:The Phn system of Mycobacterium smegmatis: a second high-affinity ABC-transporter for phosphate. Gebhard S, Tran SL, Cook GM. Microbiology. 2006 Nov;152(Pt 11):3453-65. 10.1099/mic.0.29201-0 PubMed 17074913