pX33
(Plasmid #89632)

• Purpose: Allelic change mutagenesis
• Depositing Lab: Greg Cook
• Publication: Gebhard et al Microbiology. 2006 Nov;152(Pt 11):3453-65.

Backbone

• Vector backbone: pPR23
• Backbone manufacturer: PMID: 9380741
• Backbone size w/o insert (bp): 8550
• Total vector size (bp): 9681
• Vector type: Allelic change mutagenesis
• Selectable markers: Gentamicin

Growth in Bacteria

• Bacterial Resistance(s): Gentamicin, 10 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: xylE
• Alt name: catechol 2,3-dioxygenase
• Promoter: Pmyco

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: PstI (not destroyed)
• 3′ cloning site: PstI (not destroyed)
• 5′ sequencing primer: unknown

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

SacB is used for counter-selecton in Mycobacteria to force excision and loss of this plasmid to occur.
This plasmid has been used as an allelic exchange vector. We have used SpeI site for cloning of an insert (flanking regions of a target gene to be deleted), so other groups can also use SpeI (NotI and XbaI can be used as well).