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pX33
(Plasmid #89632)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 89632 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pPR23
  • Backbone manufacturer
    PMID: 9380741
  • Backbone size w/o insert (bp) 8550
  • Total vector size (bp) 9681
  • Vector type
    Allelic change mutagenesis
  • Selectable markers
    Gentamicin

Growth in Bacteria

  • Bacterial Resistance(s)
    Gentamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    xylE
  • Alt name
    catechol 2,3-dioxygenase
  • Promoter Pmyco

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PstI (not destroyed)
  • 3′ cloning site PstI (not destroyed)
  • 5′ sequencing primer unknown
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

SacB is used for counter-selecton in Mycobacteria to force excision and loss of this plasmid to occur.
This plasmid has been used as an allelic exchange vector. We have used SpeI site for cloning of an insert (flanking regions of a target gene to be deleted), so other groups can also use SpeI (NotI and XbaI can be used as well).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pX33 was a gift from Greg Cook (Addgene plasmid # 89632 ; http://n2t.net/addgene:89632 ; RRID:Addgene_89632)
  • For your References section:

    The Phn system of Mycobacterium smegmatis: a second high-affinity ABC-transporter for phosphate. Gebhard S, Tran SL, Cook GM. Microbiology. 2006 Nov;152(Pt 11):3453-65. 10.1099/mic.0.29201-0 PubMed 17074913