PurposeBacterial expression for bzCas13b and crRNA with both HEPN domains mutated. New spacers can be cloned by digesting with BsaI.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||89901||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4245
- Total vector size (bp) 8098
Vector typeBacterial Expression
Growth in Bacteria
Growth Strain(s)NEB Stable
Copy numberLow Copy
SpeciesB. zoohelcum ATCC 43767
Insert Size (bp)3672
- Promoter Lac
- Cloning method Gibson Cloning
- 5′ sequencing primer atgccggtactgccgggcctcttgcgggat (Common Sequencing Primers)
Benchling sequence link: https://benchling.com/s/seq-gyNy5vRceFfoBk3kqStl
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBZCas13b-HEPN was a gift from Feng Zhang (Addgene plasmid # 89901 ; http://n2t.net/addgene:89901 ; RRID:Addgene_89901)
For your References section:Cas13b Is a Type VI-B CRISPR-Associated RNA-Guided RNase Differentially Regulated by Accessory Proteins Csx27 and Csx28. Smargon AA, Cox DB, Pyzocha NK, Zheng K, Slaymaker IM, Gootenberg JS, Abudayyeh OA, Essletzbichler P, Shmakov S, Makarova KS, Koonin EV, Zhang F. Mol Cell. 2017 Feb 16;65(4):618-630.e7. doi: 10.1016/j.molcel.2016.12.023. Epub 2017 Jan 5. 10.1016/j.molcel.2016.12.023 PubMed 28065598