PurposeTo express GFP-tagged Dsup in mammalian cells transiently
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||90020||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
- Promoter CMV
/ Fusion Protein
- AcGFP1 (C terminal on insert)
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Insert species is Ramazzottius varieornatus
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAcGFP1-N1-Dsup was a gift from Takekazu Kunieda (Addgene plasmid # 90020)
For your References section:Extremotolerant tardigrade genome and improved radiotolerance of human cultured cells by tardigrade-unique protein. Hashimoto T, Horikawa DD, Saito Y, Kuwahara H, Kozuka-Hata H, Shin-I T, Minakuchi Y, Ohishi K, Motoyama A, Aizu T, Enomoto A, Kondo K, Tanaka S, Hara Y, Koshikawa S, Sagara H, Miura T, Yokobori S, Miyagawa K, Suzuki Y, Kubo T, Oyama M, Kohara Y, Fujiyama A, Arakawa K, Katayama T, Toyoda A, Kunieda T. Nat Commun. 2016 Sep 20;7:12808. doi: 10.1038/ncomms12808. 10.1038/ncomms12808 PubMed 27649274
Generated by Addgene from full sequence supplied by depositor.