|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||90025||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerModified from pET-22b(+) [Park J-M, et al (2002) Biochem Biophys Res Commun 291, 23-28.]
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
- Promoter T7
/ Fusion Protein
- His (N terminal on insert)
- Cloning method Gibson Cloning
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
Insert species is Ramazzottius varieornatus
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pEThT-SAHS1 was a gift from Takekazu Kunieda (Addgene plasmid # 90025)
For your References section:Two novel heat-soluble protein families abundantly expressed in an anhydrobiotic tardigrade. Yamaguchi A, Tanaka S, Yamaguchi S, Kuwahara H, Takamura C, Imajoh-Ohmi S, Horikawa DD, Toyoda A, Katayama T, Arakawa K, Fujiyama A, Kubo T, Kunieda T. PLoS One. 2012;7(8):e44209. DOI:10.1371/journal.pone.0044209 PubMed 22937162
Generated by Addgene from full sequence supplied by depositor.
Generated by Addgene from full sequence.