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pEThT-MAHS delta MTP
(Plasmid #90032)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 90032 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEThT
  • Backbone manufacturer
    Modified from pET-22b(+) [Park J-M, et al (2002) Biochem Biophys Res Commun 291, 23-28.]
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MAHS
  • Alt name
    LC002822
  • Species
    Ramazzottius varieornatus
  • Mutation
    Deletion of mitochondria targeting peptide
  • Promoter T7
  • Tag / Fusion Protein
    • His (N terminal on insert)

Cloning Information

Resource Information

Depositor Comments

Insert species is Ramazzottius varieornatus

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEThT-MAHS delta MTP was a gift from Takekazu Kunieda (Addgene plasmid # 90032)
  • For your References section:

    Novel mitochondria-targeted heat-soluble proteins identified in the anhydrobiotic Tardigrade improve osmotic tolerance of human cells. Tanaka S, Tanaka J, Miwa Y, Horikawa DD, Katayama T, Arakawa K, Toyoda A, Kubo T, Kunieda T. PLoS One. 2015 Feb 12;10(2):e0118272. eCollection 2015. DOI:10.1371/journal.pone.0118272 PubMed 25675104