LLhG-pHG165a
(Plasmid #90039)

• Purpose: Constitutive expression of a chimeric LacI:GalR transcription repressor
• Depositing Lab: Liskin Swint-Kruse
• Publication: Meinhardt et al Proteins. 2008 Dec;73(4):941-57. doi: 10.1002/prot.22121.

Backbone

• Vector backbone: pHG165a
• Backbone manufacturer: PMID: 3012611 with lacO1 binding site of pHG165 disabled by mutation
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: LLhG
• Species: Synthetic
• Promoter: Iq

Cloning Information

• Cloning method: Ligation Independent Cloning

Resource Information

• Supplemental Documents:
  • Annotated sequence

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Second reference: PMID: 22965134. E. coli LacI DNA binding domain, LacI linker region, and E. coli GalR regulatory domain . The start codon of these genes is GTG, which is an alternative MET start codon in E. coli. The GalR insert contains an E230K mutation compared to wild type.