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pLO101
(Plasmid #90221)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 90221 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 90221-D50 50 μg of DNA in Tris buffer $495
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DNA 90221-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pCR-BluntII Topo
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Aspergillus

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ATET_07102
  • Species
    Aspergillus terreus NIH 2624
  • Promoter pLac

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The insert was cloned into this plasmid in the reverse orientation. Insert orientation does not affect plasmid function, since the insert sequences contain all sequences necessary for expression (promoter, CDS, and 3' UTR.)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLO101 was a gift from Berl Oakley (Addgene plasmid # 90221 ; http://n2t.net/addgene:90221 ; RRID:Addgene_90221)

  • For your References section:

    New multi-marker strains and complementing genes for Aspergillus nidulans molecular biology. Dohn JW Jr, Grubbs AW, Oakley CE, Oakley BR. Fungal Genet Biol. 2018 Feb;111:1-6. doi: 10.1016/j.fgb.2018.01.003. Epub 2018 Jan 5. 10.1016/j.fgb.2018.01.003 PubMed 29309843
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