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PurposeExpresses RFP-HA-DAT in mammalian cells
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Depositing Lab
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Depositing OrganizationUniversity of Pittsburgh (University, School of Medicine, and Cancer Institute)
Located in the United States of America -
Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 90265 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 90265-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 90265-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonetagRFP-C1
- Backbone size w/o insert (bp) 4725
- Total vector size (bp) 6583
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameDAT (dopamine transporter)
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SpeciesH. sapiens (human)
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Insert Size (bp)1863
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Mutationinserted HA tag (see YFP-HA-DAT)
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Entrez GeneSLC6A3 (a.k.a. DAT, DAT1, PKDYS, PKDYS1)
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site SmaI (not destroyed)
- 5′ sequencing primer NO
- 3′ sequencing primer NO
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 90265-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 90265-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
RFP-HA-DAT was a gift from Alexander Sorkin (Addgene plasmid # 90265 ; http://n2t.net/addgene:90265 ; RRID:Addgene_90265) -
For your References section:
Dopamine transporter is enriched in filopodia and induces filopodia formation. Caltagarone J, Ma S, Sorkin A. Mol Cell Neurosci. 2015 Sep;68:120-30. doi: 10.1016/j.mcn.2015.04.005. Epub 2015 Apr 30. 10.1016/j.mcn.2015.04.005 PubMed 25936602
Map uploaded by the depositor.
