1130 pBabe puroL HA FKHR H215R AAA
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||9035||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector backbonepBabe puro L
- Backbone size w/o insert (bp) 5200
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert nameFKHR H215R AAA
SpeciesH. sapiens (human)
Insert Size (bp)2000
MutationH215R T24A S256A S319A
Entrez GeneFOXO1 (a.k.a. FKH1, FKHR, FOXO1A)
/ Fusion Protein
- HA (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer pBabe5 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Oligonucleotides 5'-GCGCGGATCCATGGCCGAGGCGCCTCAGGTG-3' and 5'-CGCGCTCGAGGAATTCTCAGCCTGACACCCAGCTATG-3' were used to PCR amplify the FKHR cDNA. The PCR products, restricted with BamHI and XhoI, were ligated to similarly restricted pBABE-puroL to give pBABE-puroL-FKHR.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:1130 pBabe puroL HA FKHR H215R AAA was a gift from William Sellers (Addgene plasmid # 9035 ; http://n2t.net/addgene:9035 ; RRID:Addgene_9035)
For your References section:A novel mechanism of gene regulation and tumor suppression by the transcription factor FKHR. Ramaswamy S, Nakamura N, Sansal I, Bergeron L, Sellers WR. Cancer Cell. 2002 Jul . 2(1):81-91. 10.1016/S1535-6108(02)00086-7 PubMed 12150827
Map uploaded by the depositor.