Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pADH99
(Plasmid #90979)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 90979 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pUC19
  • Backbone size w/o insert (bp) 2242
  • Total vector size (bp) 11119
  • Vector type
    CRISPR
  • Selectable markers
    Nourseothricin (NAT)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    US_HIS1
  • Insert Size (bp)
    433

Gene/Insert 2

  • Gene/Insert name
    FRT
  • Insert Size (bp)
    34

Gene/Insert 3

  • Gene/Insert name
    pENO1
  • Insert Size (bp)
    783

Gene/Insert 4

  • Gene/Insert name
    C. albicans Cas9
  • Insert Size (bp)
    4113

Gene/Insert 5

  • Gene/Insert name
    pMAL2
  • Insert Size (bp)
    548

Gene/Insert 6

  • Gene/Insert name
    FLP
  • Insert Size (bp)
    1272

Gene/Insert 7

  • Gene/Insert name
    NAT 1 of 2
  • Insert Size (bp)
    846

Resource Information

  • Supplemental Documents
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pADH99 was a gift from Aaron Hernday (Addgene plasmid # 90979 ; http://n2t.net/addgene:90979 ; RRID:Addgene_90979)
  • For your References section:

    An efficient, rapid, and recyclable system for CRISPR-mediated genome editing in Candida albicans. Nguyen N, Quail MMF, Hernday AD . mSphere Apr 2017, 2 (2) e00149-17 10.1128/mSphereDirect.00149-17