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mApple-Sec61b-C1
(Plasmid #90993)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 90993 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4732
  • Total vector size (bp) 5000
  • Modifications to backbone
    Cloned from pAc-GFPC1-Sec61beta (Addgene # 15108) through AgeI and BsrG1 sites to change the color of the fluorescent protein.
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mApple-Sec61b
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    1014
  • Entrez Gene
    SEC61B
  • Promoter CMV
  • Tag / Fusion Protein
    • mApple (red fluorescence) (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (not destroyed)
  • 3′ cloning site BsrG1 (not destroyed)
  • 5′ sequencing primer CMVfor
  • 3′ sequencing primer SV40polyA
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • Addgene Notes
  • A portion of this plasmid was derived from a plasmid made by
    The plasmid is modified from pAcGFP-C1-Sec61b, generated by Tom Rapoport's Lab (Harvard). It was acquired by Craig Blackstone's Lab (NIH\NINDS), and modified in Jennifer Lippincott-Schwartz's Lab (NIH\NICHD).
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mApple-Sec61b-C1 was a gift from Jennifer Lippincott-Schwartz (Addgene plasmid # 90993 ; http://n2t.net/addgene:90993 ; RRID:Addgene_90993)
  • For your References section:

    Increased spatiotemporal resolution reveals highly dynamic dense tubular matrices in the peripheral ER. Nixon-Abell J, Obara CJ, Weigel AV, Li D, Legant WR, Xu CS, Pasolli HA, Harvey K, Hess HF, Betzig E, Blackstone C, Lippincott-Schwartz J. Science. 2016 Oct 28;354(6311). pii: aaf3928. Epub 2016 Oct 27. 10.1126/science.aaf3928 PubMed 27789813