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(Plasmid #91946)


Item Catalog # Description Quantity Price (USD)
Plasmid 91946 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    K. Deisseroth Lab (Addgene #37084)
  • Backbone size w/o insert (bp) 6342
  • Total vector size (bp) 6484
  • Modifications to backbone
    1. The EF1α promoter between the restriction sites MluI and BamHI in the backbone plasmid was replaced by the CBh promoter, which was PCR-amplified from the plasmid pX330-U6-Chimeric_BB-CBh-hSpCas9 (Addgene #42230). 2. The WPRE-pA between EcoRI and BstEII sites in the backbone plasmid was replaced by the CW3SL sequence, which was amplified by PCR from the plasmid pAAV-CW3SL-EGFP (Addgene #61463). 3. The mouse Cnr2 gene was PCR-amplified from the plasmid MR205238L2 (Origene) with primers containing the sequences of FLAG and P2A, and then, inserted into the NheI (BmtI) site downstream of the eGFP sequence in the control plasmid.
  • Vector type

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number

Gene/Insert 1

  • Gene/Insert name
  • Species
  • Insert Size (bp)
  • Tag / Fusion Protein
    • None

Gene/Insert 2

  • Gene/Insert name
    type-2 cannabinoid receptor
  • Alt name
    CB2 cannabinoid receptor
  • Alt name
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
  • Tag / Fusion Protein
    • FLAG

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-CBh-DIO-CB2-EGFP was a gift from Jimok Kim (Addgene plasmid # 91946 ; ; RRID:Addgene_91946)
  • For your References section:

    Distinct roles of neuronal and microglial CB2 cannabinoid receptors in the mouse hippocampus. Li Y, Kim J. Neuroscience. 2017 Sep 6. pii: S0306-4522(17)30629-2. doi: 10.1016/j.neuroscience.2017.08.053. 10.1016/j.neuroscience.2017.08.053 PubMed 28888955