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CRY-Gal∆DD (B1013)
(Plasmid #92035)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 92035 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 92035-D50 50 μg of DNA in Tris buffer $495
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DNA 92035-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pmCherryC1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 5270
  • Total vector size (bp) 7373
  • Modifications to backbone
    IRES element between end of mCherry and SacI sites
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CRY2
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
    2103
  • Mutation
    Fusion of plant CRY2 to Gal4 binding domain (AA1-65), downstream of mCherry-IRES element
  • Entrez Gene
    CRY2 (a.k.a. AT1G04400, AT-PHH1, ATCRY2, CRYPTOCHROME 2 APOPROTEIN, F19P19.14, F19P19_14, FHA, PHH1, cryptochrome 2)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI, SacI (not destroyed)
  • 3′ cloning site Not1 between linker of CRY2 and Gal4 BD (not destroyed)
  • 5′ sequencing primer custom
  • 3′ sequencing primer custom
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CRY-Gal∆DD (B1013) was a gift from Chandra Tucker (Addgene plasmid # 92035 ; http://n2t.net/addgene:92035 ; RRID:Addgene_92035)

  • For your References section:

    Bidirectional approaches for optogenetic regulation of gene expression in mammalian cells using Arabidopsis cryptochrome 2. Pathak GP, Spiltoir JI, Hoglund C, Polstein LR, Heine-Koskinen S, Gersbach CA, Rossi J, Tucker CL. Nucleic Acids Res. 2017 Apr 20. doi: 10.1093/nar/gkx260. 10.1093/nar/gkx260 PubMed 28431041
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