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(Plasmid #92266)


Item Catalog # Description Quantity Price (USD)
Plasmid 92266 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 5445
  • Total vector size (bp) 9619
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    dGW182 6W6A
  • Alt name
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
  • Mutation
    The following point mutations were introduced W239A; W293A; W316A; W338A; W438A; W484A.
  • GenBank ID
  • Entrez Gene
    gw (a.k.a. Dmel_CG31992, CG11484, CG31992, CG9905, Dmel\CG31992, GQ182, GW, GW182, Gw, Gw182, anon-EST:Posey205, dGW182, gw182)
  • Promoter Ac5
  • Tag / Fusion Protein
    • NHA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SbfI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer ACA CAA AGC CGC TCC ATC AG
  • 3′ sequencing primer CCT TAG AAG GCA CAG TCG AGG
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

Note: Addgene's quality control sequencing finds two additional amino acid residue substitutions (V704A and N799S). The effects of these additional residue changes on protein function are not known.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMCh-623 was a gift from Marina Chekulaeva & Witold Filipowicz (Addgene plasmid # 92266 ; ; RRID:Addgene_92266)
  • For your References section:

    miRNA repression involves GW182-mediated recruitment of CCR4-NOT through conserved W-containing motifs. Chekulaeva M, Mathys H, Zipprich JT, Attig J, Colic M, Parker R, Filipowicz W. Nat Struct Mol Biol. 2011 Oct 7;18(11):1218-26. doi: 10.1038/nsmb.2166. 10.1038/nsmb.2166 PubMed 21984184