Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

(Plasmid #92287)


Item Catalog # Description Quantity Price (USD)
Plasmid 92287 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 5459
  • Total vector size (bp) 6329
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    dGW182 NED
  • Alt name
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
  • Mutation
    dGW182 NED: aa 205-490
  • GenBank ID
  • Entrez Gene
    gw (a.k.a. Dmel_CG31992, CG11484, CG31992, CG9905, Dmel\CG31992, GQ182, GW, GW182, Gw, Gw182, anon-EST:Posey205, dGW182, gw182)
  • Promoter Ac5
  • Tag / Fusion Protein
    • NHA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SbfI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer ACA CAA AGC CGC TCC ATC AG
  • 3′ sequencing primer CCT TAG AAG GCA CAG TCG AGG
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMCh-139 was a gift from Marina Chekulaeva & Witold Filipowicz (Addgene plasmid # 92287 ; ; RRID:Addgene_92287)
  • For your References section:

    The GW/WG repeats of Drosophila GW182 function as effector motifs for miRNA-mediated repression. Chekulaeva M, Parker R, Filipowicz W. Nucleic Acids Res. 2010 Oct;38(19):6673-83. doi: 10.1093/nar/gkq501. Epub 2010 Jun 8. 10.1093/nar/gkq501 PubMed 20530530