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pTRE3G-GSX2
(Plasmid #96964)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 96964 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 96964-D50 50 μg of DNA in Tris buffer $495
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DNA 96964-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pTRE3G-IRES
  • Backbone manufacturer
    Clontech
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GSX2
  • Alt name
    GSH2
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Gsx2 (a.k.a. Gsh-2, Gsh2)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTRE3G-GSX2 was a gift from Elena Cattaneo (Addgene plasmid # 96964 ; http://n2t.net/addgene:96964 ; RRID:Addgene_96964)

  • For your References section:

    Differentiation of human telencephalic progenitor cells into MSNs by inducible expression of Gsx2 and Ebf1. Faedo A, Laporta A, Segnali A, Galimberti M, Besusso D, Cesana E, Belloli S, Moresco RM, Tropiano M, Fuca E, Wild S, Bosio A, Vercelli AE, Biella G, Cattaneo E. Proc Natl Acad Sci U S A. 2017 Feb 14;114(7):E1234-E1242. doi: 10.1073/pnas.1611473114. Epub 2017 Jan 30. 10.1073/pnas.1611473114 PubMed 28137879
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