pBAD-FimH-9xHis
(Plasmid
#97305)
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PurposeE.coli UTI89 FimH signal peptide and lectin domain
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 97305 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepBAD
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 4000
- Total vector size (bp) 4566
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameType 1 fimbiral adhesin FimH
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Alt nameFimH
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SpeciesSynthetic
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Insert Size (bp)567
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Tag
/ Fusion Protein
- 9xHis (C terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer pBAD forward primer
- 3′ sequencing primer pBAD reverse primer (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBAD-FimH-9xHis was a gift from Per Svenningsen (Addgene plasmid # 97305 ; http://n2t.net/addgene:97305 ; RRID:Addgene_97305)
Map uploaded by the depositor.
